Culdocentesis is a procedure in which peritoneal fluid is obtained from the cul de sac of a female patient. It involves the introduction of a spinal needle through the vaginal wall into the peritoneal space of the pouch of Douglas. Culdocentesis is a medical procedure involving the extraction of fluid from the pouch of Douglas (a rectouterine pouch[posterior to the vagina) through a needle. It can be one diagnostic technique used in identifying pelvic inflammatory disease (in which case purulent fluid will be extracted) and ruptured ectopic pregnancies that cause hemoperitoneum. In the procedure, the rectouterine pouch is often reached through the posterior fornix of the vagina. The process of creating the hole is called “colpotomy” if a scalpel incision is made to drain the fluid rather than using a needle
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